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ALKBH5 Polyclonal antibody

ALKBH5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

IHC, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 16837-1-AP

Print datasheet

Synonyms

ABH5, ALKBH5, OFOXD, OFOXD1



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ALKBH5 Ready-To-Use IHC Kit. ALKBH5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16837-1-AP targets ALKBH5 in WB, RIP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited Applications CoIP, IF, IHC, RIP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, pig, bovine
Immunogen ALKBH5 fusion protein Ag10516 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alkB, alkylation repair homolog 5 (E. coli)
Synonyms ABH5, ALKBH5, OFOXD, OFOXD1
Calculated Molecular Weight 458 aa, 51 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC062339
Gene Symbol ALKBH5
Gene ID (NCBI) 54890
RRIDAB_2242665
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P6C2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.

实验方案

Product Specific Protocols
WB protocol for ALKBH5 antibody 16837-1-APDownload protocol
IHC protocol for ALKBH5 antibody 16837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Genet

N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.

Authors - Yuan Li
humanIF

Cell Res

The m6A methylome of SARS-CoV-2 in host cells.

Authors - Jun'e Liu
  • KD Validated
mouseWB,RIP

Nat Cell Biol

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.

Authors - Xiang-Qian Gao
humanWB

Nat Commun

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis

Authors - Jian Zhong
humanWB

Mol Cell

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Authors - Elodie Villa
humanRIP

Mol Cancer

Demethylase ALKBH5 suppresses invasion of gastric cancer via PKMYT1 m6A modification.

Authors - Yiyang Hu