CoraLite® Plus 488-conjugated ARL13B Monoclonal antibody

ARL13B Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Canine

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H6C3

Cat No : CL488-66739

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Synonyms

ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8



经过测试的应用

Positive IF detected inhTERT-RPE1 cells, MDCK cells
For other applications, we recommend the unconjugated version of this antibody, 66739-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66739 targets ARL13B in IF applications and shows reactivity with Human, Canine samples.

Tested Applications IF
Tested Reactivity Human, Canine
Immunogen ARL13B fusion protein Ag12031 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ADP-ribosylation factor-like 13B
Synonyms ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC094725
Gene Symbol ARL13B
Gene ID (NCBI) 200894
RRIDAB_2883377
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ3SXY8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ARL13B antibody CL488-66739Download protocol
Standard Protocols
Click here to view our Standard Protocols