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- KD/KO Validated
CD44 Polyclonal antibody
CD44 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
283
验证数据展示
经过测试的应用
Positive WB detected in | MDA-MB-231 cells, HeLa cells, HUVEC cells, A549 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human lung cancer tissue, human colon cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, human colon cancer tissue, A375 cells, HUVEC cells, UCMSCs cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15675-1-AP targets CD44 in WB, IP, IF, FC, IHC, CoIP, ELISA, Neutralization applications and shows reactivity with human samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, FC, IHC, CoIP, Neutralization |
Tested Reactivity | human |
Cited Reactivity | human, sika deer, Bovine |
Immunogen | CD44 fusion protein Ag8143 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CD44 molecule (Indian blood group) |
Synonyms | CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I |
Calculated Molecular Weight | 742 aa, 82 kDa |
Observed Molecular Weight | 80-95 kDa |
GenBank Accession Number | BC004372 |
Gene Symbol | CD44 |
Gene ID (NCBI) | 960 |
ENSEMBL Gene ID | ENSG00000026508 |
RRID | AB_2076198 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P16070 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CD44 antibody 15675-1-AP | Download protocol |
IHC protocol for CD44 antibody 15675-1-AP | Download protocol |
IF protocol for CD44 antibody 15675-1-AP | Download protocol |
IP protocol for CD44 antibody 15675-1-AP | Download protocol |
FC protocol for CD44 antibody 15675-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) The Critical Role of The Piezo1/β-catenin/ATF4 Axis on The Stemness of Gli1+ BMSCs During Simulated Microgravity-Induced Bone Loss | ||
Int J Oral Sci LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma | ||
Acta Pharm Sin B Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy | ||
Biomaterials Hyaluronan decorated layer-by-layer assembled lipid nanoparticles for miR-181a delivery in glioblastoma treatment |