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Caspase 7/p20 Polyclonal antibody

Caspase 7/p20 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 27155-1-AP

Print datasheet

Synonyms

Apoptotic protease Mch 3, CASP 7, CASP7, Caspase 7, Caspase 7/p20, Caspase7, CMH 1, ICE LAP3, ICE like apoptotic protease 3, MCH3



经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, LNCaP cells, MCF-7 cells, mouse liver tissue, PC-3 cells
Positive IP detected inHEK-293 cells
Positive IF detected inMCF-7 cells, mouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy CASP7 Ready-To-Use IHC Kit. CASP7 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27155-1-AP targets Caspase 7/p20 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF, IP, WB, ELISA
Cited Applications IHC, IP, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen Caspase 7/p20 fusion protein Ag25904 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caspase 7, apoptosis-related cysteine peptidase
Synonyms Apoptotic protease Mch 3, CASP 7, CASP7, Caspase 7, Caspase 7/p20, Caspase7, CMH 1, ICE LAP3, ICE like apoptotic protease 3, MCH3
Calculated Molecular Weight 303 aa, 34 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC015799
Gene Symbol CASP7
Gene ID (NCBI) 840
RRIDAB_2880779
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55210
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.

实验方案

Product Specific Protocols
WB protocol for Caspase 7/p20 antibody 27155-1-APDownload protocol
IF protocol for Caspase 7/p20 antibody 27155-1-APDownload protocol
IP protocol for Caspase 7/p20 antibody 27155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Nat Commun

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Authors - Mengyan Hu
humanWB

J Pineal Res

Melatonin synergizes the chemotherapeutic effect of 5-fluorouracil in colon cancer by suppressing PI3K/AKT and NF-κB/iNOS signaling pathways.

Authors - Yue Gao
humanWB

Cell Rep

Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation

Authors - Zehe Ge
humanWB

Front Immunol

Identification of molecular subtypes based on PANoptosis-related genes and construction of a signature for predicting the prognosis and response to immunotherapy response in hepatocellular carcinoma

Authors - Jinfeng Zhu
humanWB

Phytother Res

Targeting HSP90 with picropodophyllin suppresses gastric cancer tumorigenesis by disrupting the association of HSP90 and AKT

Authors - Xiaoli Li
humanWB

J Agric Food Chem

β-Sitosterol-D-glucoside from sweet potato exerts an anti-breast cancer activity by upregulating microRNA-10a and PI3K/Akt signaling pathway.

Authors - Heshan Xu