GST Tag Monoclonal antibody

GST Tag Monoclonal Antibody for IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Recombinant protein, Schistosoma japonicum and More (2)

Applications

IP, WB, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

3G12B10

Cat No : 66001-2-Ig

Print datasheet

Synonyms

GST, GST Tag



经过测试的应用

Positive WB detected inRecombinant protein
Positive IP detected inE. coli lysate

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66001-2-Ig targets GST Tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with Recombinant protein, Schistosoma japonicum samples.

Tested Applications IP, WB, ELISA
Cited Applications CoIP, IF, IP, WB
Tested Reactivity Recombinant protein, Schistosoma japonicum
Cited Reactivityhuman, mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name GST Tag
Synonyms GST, GST Tag
Calculated Molecular Weight 26 kDa
GenBank Accession NumberFN315687
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881488
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the N- or C-terminus of targeted proteins.

实验方案

Product Specific Protocols
WB protocol for GST Tag antibody 66001-2-IgDownload protocol
IP protocol for GST Tag antibody 66001-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cancer

METTL16 antagonizes MRE11-mediated DNA end resection and confers synthetic lethality to PARP inhibition in pancreatic ductal adenocarcinoma

Authors - Xiangyu Zeng
WB

Nat Cell Biol

Phagolysosome resolution requires contacts with the endoplasmic reticulum and phosphatidylinositol-4-phosphate signalling.

Authors - Roni Levin-Konigsberg
WB

Sci Bull (Beijing)

Targeted clearance of mitochondria by an autophagy-tethering compound (ATTEC) and its potential therapeutic effects

Authors - Shuixia Tan
WB

Nat Commun

Fungal secondary metabolism is governed by an RNA-binding protein CsdA/RsdA complex

Authors - Zili Song
WB

Nat Commun

N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization

Authors - Weiji Weng
WB,IP

Adv Sci (Weinh)

Elevated Nuclear PHGDH Synergistically Functions with cMyc to Reshape the Immune Microenvironment of Liver Cancer

Authors - Hongwen Zhu