IL-2 Monoclonal antibody

IL-2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F11C5

Cat No : 60306-1-Ig

Print datasheet

Synonyms

IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF



经过测试的应用

Positive WB detected inJurkat cells, MOLT-4 cells, human peripheral blood leukocyte
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60306-1-Ig targets IL-2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIHC, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, pig
Immunogen IL-2 fusion protein Ag10199 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name interleukin 2
Synonyms IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF
Calculated Molecular Weight 153 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC070338
Gene Symbol IL2
Gene ID (NCBI) 3558
RRIDAB_2881420
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP60568
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.

实验方案

Product Specific Protocols
WB protocol for IL-2 antibody 60306-1-IgDownload protocol
IHC protocol for IL-2 antibody 60306-1-IgDownload protocol
IF protocol for IL-2 antibody 60306-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Transl Psychiatry

A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.

Authors - Yinrui Guo
mouseWB

PLoS One

N-Acetylcysteine Attenuates Ischemia-Reperfusion-Induced Apoptosis and Autophagy in Mouse Liver via Regulation of the ROS/JNK/Bcl-2 Pathway.

Authors - Chengfen Wang
mouseWB

Inflammation

Links Between Gut Dysbiosis and Neurotransmitter Disturbance in Chronic Restraint Stress-Induced Depressive Behaviours: the Role of Inflammation

Authors - Hai‑long Yang
pigWB,IHC

Food Addit Contam Part A Chem Anal

FumDSB can alleviate the inflammatory response induced by fumonisin B1 in growing pigs.

Authors - Quancheng Liu
humanIHC

J Clin Exp Hematop

Role of TGF-beta1 and TNF-alpha1 produced by neoplastic cells in the pathogenesis of fibrosis in patients with hematologic neoplasms

Authors - Haruko Takizawa
humanWB

Front Immunol

PTPRC promoted CD8+ T cell mediated tumor immunity and drug sensitivity in breast cancer: based on pan-cancer analysis and artificial intelligence modeling of immunogenic cell death-based drug sensitivity stratification

Authors - Pengping Li