• Featured Product
  • KD/KO Validated

KMO Polyclonal antibody

KMO Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 10698-1-AP

Print datasheet

Synonyms

Kynurenine 3-monooxygenase, Kynurenine 3-hydroxylase, Kynurenine 3 monooxygenase, Kynurenine 3 hydroxylase, EC:1.14.13.9



经过测试的应用

Positive IHC detected inhuman kidney tissue, human prostate cancer tissue, human breast cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10698-1-AP targets KMO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen KMO fusion protein Ag1109 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
Synonyms Kynurenine 3-monooxygenase, Kynurenine 3-hydroxylase, Kynurenine 3 monooxygenase, Kynurenine 3 hydroxylase, EC:1.14.13.9
Calculated Molecular Weight486 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC005297
Gene Symbol KMO
Gene ID (NCBI) 8564
RRIDAB_2296744
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15229
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).

实验方案

Product Specific Protocols
WB protocol for KMO antibody 10698-1-APDownload protocol
IHC protocol for KMO antibody 10698-1-APDownload protocol
IF protocol for KMO antibody 10698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

J Hazard Mater

Particulate matter-induced metabolic recoding of epigenetics in macrophages drives pathogenesis of chronic obstructive pulmonary disease

Authors - Myungkyung Noh
humanWB

Circ Res

Activation of NAD(P)H Oxidase by Tryptophan-Derived 3-Hydroxykynurenine Accelerates Endothelial Apoptosis and Dysfunction In Vivo.

Authors - Qiongxin Wang
human, mouseIF

J Am Soc Nephrol

Loss of Kynurenine 3-Mono-oxygenase Causes Proteinuria.

Authors - Ron Korstanje
mouseIHC,IF

J Transl Med

Identification and experimental validation of KMO as a critical immune-associated mitochondrial gene in unstable atherosclerotic plaque

Authors - Fu-Jun Liao
humanIHC

J Intern Med

Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans.

Authors - R Baumgartner
mouseWB

J Affect Disord

Coprococcus eutactus screened from healthy adolescent attenuates chronic restraint stress-induced depression-like changes in adolescent mice: Potential roles in the microbiome and neurotransmitter modulation

Authors - Liuting Xu