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PCNA Monoclonal antibody

PCNA Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat and More (2)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

10D10E11

Cat No : 60097-1-Ig

Print datasheet

Synonyms

Cyclin, PCNA



经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, C6 cells, ROS1728 cells, NIH/3T3 cells, 3T3-L1 cells, 4T1 cells, RAW 264.7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman gliomas tissue, human liver cancer tissue, human breast cancer tissue, mouse ovary tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, mouse heart tissue
Positive FC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 750 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:250-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60097-1-Ig targets PCNA in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman, mouse, rat, rabbit, zebrafish, pig
Immunogen PCNA fusion protein Ag7416 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proliferating cell nuclear antigen
Synonyms Cyclin, PCNA
Calculated Molecular Weight 29 kDa/31 kDa
Observed Molecular Weight 36-38 kDa
GenBank Accession NumberBC000491
Gene Symbol PCNA
Gene ID (NCBI) 5111
RRIDAB_2236728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP12004
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

实验方案

Product Specific Protocols
WB protocol for PCNA antibody 60097-1-IgDownload protocol
IHC protocol for PCNA antibody 60097-1-IgDownload protocol
IF protocol for PCNA antibody 60097-1-IgDownload protocol
IP protocol for PCNA antibody 60097-1-IgDownload protocol
FC protocol for PCNA antibody 60097-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization.

Authors - Shangda Yang
humanWB

Nat Commun

Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing.

Authors - Chunjie Sheng
pigWB

Microbiome

Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.

Authors - Leli Wang
mouse,ratWB,IF

Environ Pollut

Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling

Authors - Yifan Hong
mouseIHC

Biofabrication

Scale-out production of extracellular vesicles derived from natural killer cells via mechanical stimulation in a seesaw-motion bioreactor for cancer therapy.

Authors - Jianguo Wu
mouseIHC

JCI Insight

RNF112-mediated FOXM1 ubiquitination suppresses the proliferation and invasion of gastric cancer

Authors - Shengwei Zhang