V5-tag Polyclonal antibody

V5-tag Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (2)

Applications

IP, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 14440-1-AP

Print datasheet

Synonyms

GKPIPNPLLGLDST, V5, V5 tag, V5-tag



经过测试的应用

Positive WB detected inRecombinant protein
Positive IP detected inRecombinant protein protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14440-1-AP targets V5-tag in WB, RIP, IP, IF, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications IP, WB, ELISA
Cited Applications ChIP, IF, IP, RIP, WB
Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name V5-tag
Synonyms GKPIPNPLLGLDST, V5, V5 tag, V5-tag
Calculated Molecular Weight 1 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2878059
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.

实验方案

Product Specific Protocols
WB protocol for V5-tag antibody 14440-1-APDownload protocol
IP protocol for V5-tag antibody 14440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

J Extracell Vesicles

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

Authors - Xiaohan Gao
WB

Sci Adv

The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals

Authors - Liuke Sun
IF

Nat Commun

Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.

Authors - Xianding Sun
WB

Nucleic Acids Res

HSP90 and Aha1 modulate microRNA maturation through promoting the folding of Dicer1.

Authors - Xiaochuan Liu
humanIP,WB

Nucleic Acids Res

CGG repeats trigger translational frameshifts that generate aggregation-prone chimeric proteins.

Authors - Shannon E Wright
WB

Cell Death Differ

Mitophagy protein PINK1 suppresses colon tumor growth by metabolic reprogramming via p53 activation and reducing acetyl-CoA production.

Authors - Kunlun Yin