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YY1 Monoclonal antibody

YY1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, monkey and More (1)

Applications

IF, IHC, IP, WB, ELISA and More (3)

Conjugate

Unconjugated

CloneNo.

2E11C5

Cat No : 66281-1-Ig

Print datasheet

Synonyms

DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor



经过测试的应用

Positive WB detected inC6 cells, A431 cells, MCF-7 cells, NIH/3T3 cells, HeLa cells, COS-7 cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy YY1 Ready-To-Use IHC Kit. YY1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite® Plus 750 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66281-1-Ig targets YY1 in WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications ChIP, CoIP, IF, IHC, IP, RIP, WB
Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat, pig
Immunogen YY1 fusion protein Ag17732 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name YY1 transcription factor
Synonyms DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor
Calculated Molecular Weight 414 aa, 45 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC037308
Gene Symbol YY1
Gene ID (NCBI) 7528
RRIDAB_2881664
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25490
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.

实验方案

Product Specific Protocols
WB protocol for YY1 antibody 66281-1-IgDownload protocol
IHC protocol for YY1 antibody 66281-1-IgDownload protocol
IF protocol for YY1 antibody 66281-1-IgDownload protocol
IP protocol for YY1 antibody 66281-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Cell

Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of Transcription.

Authors - Rui Xiao
mouseWB

Nat Commun

miR-29b contributes to multiple types of muscle atrophy.

Authors - Jin Li
humanWB,IF,IP

Oncogene

AKIP1 accelerates glioblastoma progression through stabilizing EGFR expression

Authors - Sicheng Wan
mouseWB

Mol Ther Nucleic Acids

Angiotensin II-induced muscle atrophy via PPARγ suppression is mediated by miR-29b.

Authors - Jin Li
humanWB

Cell Death Dis

CircHIPK3 promotes colorectal cancer growth and metastasis by sponging miR-7.

Authors - Kaixuan Zeng
humanWB

Cancers (Basel)

MiR-302b as a Combinatorial Therapeutic Approach to Improve Cisplatin Chemotherapy Efficacy in Human Triple-Negative Breast Cancer.

Authors - Alessandra Cataldo