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FUS/TLS Polyclonal antibody

FUS/TLS Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, IP, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 11570-1-AP

Print datasheet

Synonyms

75 kDa DNA pairing protein, FUS, FUS1, FUS-CHOP, hnRNP P2, Oncogene FUS, Oncogene TLS, POMp75, RNA binding protein FUS, TLS, TLS/CHOP



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IP detected inK-562 cells
Positive IHC detected inmouse brain tissue, human ovary tumor tissue, rat brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse colon tissue, HepG2 cells, HeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FUS/TLS Ready-To-Use IHC Kit. FUS/TLS primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11570-1-AP targets FUS/TLS in WB, RIP, IP, IHC, IF, FC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications chIP, CoIP, IF, IHC, IP, RIP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen FUS/TLS fusion protein Ag2150 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fusion (involved in t(12;16) in malignant liposarcoma)
Synonyms 75 kDa DNA pairing protein, FUS, FUS1, FUS-CHOP, hnRNP P2, Oncogene FUS, Oncogene TLS, POMp75, RNA binding protein FUS, TLS, TLS/CHOP
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 68-75 kDa
GenBank Accession NumberBC026062
Gene Symbol FUS
Gene ID (NCBI) 2521
RRIDAB_2247082
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS. FUS was detected double bands of 68-74 kDa (PMID:31519807).

实验方案

Product Specific Protocols
WB protocol for FUS/TLS antibody 11570-1-APDownload protocol
IHC protocol for FUS/TLS antibody 11570-1-APDownload protocol
IF protocol for FUS/TLS antibody 11570-1-APDownload protocol
IP protocol for FUS/TLS antibody 11570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
humanIHC,IF

Nature

Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia.

Authors - Deng Han-Xiang HX
human,mouseWB

Nat Med

Antisense oligonucleotide silencing of FUS expression as a therapeutic approach in amyotrophic lateral sclerosis.

Authors - Vladislav A Korobeynikov
humanWB,IF

Cell

Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains.

Authors - Lin Guo
humanWB

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
mouseWB

Nat Neurosci

FUS-mediated regulation of acetylcholine receptor transcription at neuromuscular junctions is compromised in amyotrophic lateral sclerosis.

Authors - Gina Picchiarelli
  • KD Validated