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  • Featured Product
  • KD/KO Validated

GRP78/BIP Polyclonal antibody

GRP78/BIP Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

FC, IF/ICC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11587-1-AP

Print datasheet

Synonyms

BIP, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSP A5, HSPA5, MIF2

验证数据展示

  • WB analysis using 11587-1-AP

    WB analysis using 11587-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis using 11587-1-AP

    WB analysis using 11587-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11587-1-AP

    IHC staining of human pancreas cancer using 11587-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GRP78/HSPA5 antibody included
  • FC experiment of HeLa using 11587-1-AP

    FC experiment of HeLa using 11587-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GRP78/BIP (11587-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IP experiment of MCF-7 using 11587-1-AP

    IP experiment of MCF-7 using 11587-1-AP

    IP result of anti-GRP78/BIP (IP:11587-1-AP, 3ug; Detection:11587-1-AP 1:1000) with MCF-7 cells lysate 3250ug.

  • WB analysis using 11587-1-AP

    WB analysis using 11587-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 11587-1-AP

    IF Staining of HepG2 using 11587-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRP78/BIP antibody (11587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inBxPC-3 cells, Caco-2 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue, HT-29 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue, human breast cancer tissue, mouse kidney tissue, rat brain tissue, mouse heart tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GRP78/HSPA5 Ready-To-Use IHC Kit. GRP78/HSPA5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11587-1-AP targets GRP78/BIP in WB,IP,IHC,IF,FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB,IP,IHC,IF,CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, deer, chicken, carp, medaka embryos, monkey, fish, zebrafish
Immunogen GRP78/BIP fusion protein Ag2188 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Synonyms BIP, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSP A5, HSPA5, MIF2
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 70-78 kDa
GenBank Accession NumberBC020235
Gene Symbol HSPA5
Gene ID (NCBI) 3309
RRIDAB_2119855
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11021
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.

实验方案

Product Specific Protocols
WB protocol for GRP78/BIP antibody 11587-1-APDownload protocol
IHC protocol for GRP78/BIP antibody 11587-1-APDownload protocol
IF protocol for GRP78/BIP antibody 11587-1-APDownload protocol
IP protocol for GRP78/BIP antibody 11587-1-APDownload protocol
FC protocol for GRP78/BIP antibody 11587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Science

Translation from the 5' untranslated region shapes the integrated stress response.

Authors - Shelley R Starck
View Article
mouseWB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
View Article
mouseWB

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
View Article
mouseWB

Crit Care

Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.

Authors - Lingbing Zhang
View Article
human,ratWB

Acta Pharm Sin B

SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.

Authors - Fen Long
View Article
human,mouseWB

J Pineal Res

Melatonin alleviates PM2.5 -induced glucose metabolism disorder and lipidome alteration by regulating endoplasmic reticulum stress

Authors - Zhou Du
View Article