Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB result of HO-1/HMOX1 antibody (66743-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HO-1/HMOX1 transfected HeLa cells.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HO-1/HMOX1 (66743-1-Ig, Clone:2D10A5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using HO-1/HMOX1 antibody (66743-1-Ig, Clone: 2D10A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.