GMP-grade recombinant human HGF (HZ-1084-GMP) stimulates dose-dependent proliferation of the 4MBr-5 Monkey epithelial cell line. Viable cell number was quantitiatively assessed by PrestoBlue Cell Viability Reagent. 4MBr-5 cells were treated with increasing concentrations of recombinant human HGF for 120 hours. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 value ranges from 5-25 ng/mL.