Purity of recombinant human FLT3 Ligand was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human FLT3 (HZ-1151) Ligand stimulates dose-dependent proliferation of the human acute myeloid leukemia cell line (OCI-AML5). Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. OCI-AML5 cells were treated with increasing concentrations of recombinant human FLT3 Ligand for 72 hours. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range is 0.4-3 ng/mL. 

Purity of GMP-grade recombinant human FLT3 Ligand was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP-grade recombinant human FLT3 Ligand (HZ-1151-GMP) stimulates dose-dependent proliferation of the human acute myeloid leukemia cell line (OCI-AML5). Viable cell number was quantitiatively assessed by PrestoBlue Cell Viability Reagent. OCI-AML5 cells were treated with increasing concentrations of recombinant human FLT3 Ligand for 72 hours.The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 value ranges from 0.4-3.0 ng/mL.