Purity of recombinant human IFN beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
Recombinant human IFN beta (HZ-1298) dose-dependently inhibits growth of the TF-1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IFN beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.015-0.08 ng/mL.
Proteintech IFN beta (HZ-1298) demonstrates equivalent induction of proliferation and EC50 to leading competitors. IFN beta dose-dependently inhibits growth of the TF-1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IFN beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.015-0.08 ng/mL.