Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB result of IBA1 antibody (10904-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and IBA1 knockout THP-1 cells.
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using IBA1 antibody (10904-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human IBA1 (10904-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IP result of anti-IBA1 (IP:10904-1-AP, 4ug; Detection:10904-1-AP 1:500) with THP-1 cells lysate 2400 ug.
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).