Various lysate were subjected to SDS PAGE followed by western blot with 11724-1-AP (LIN28 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11724-1-AP (LIN28 antibody) at dilution of 1:50 (under 40x lens).
Confocal immunofluorescent analysis of human embronic stem cells with 11724-1-AP at dilution of 1:200. The PE shows staining with 11724-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI. (10X).
Confocal immunofluorescent analysis of human embronic stem cells with 11724-1-AP at dilution of 1:400. The PE shows staining with 11724-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI. (20X).
WB data of LIN28 antibody (11724-1-AP, 1:500) with si-control and si-LIN28 transfected NCCIT cells.
IP result of anti-LIN28(IP:11724-1-AP, 4ug; Detection:11724-1-AP 1:2000) with K-562 cells lysate 1360 ug.