Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15215-1-AP (CNPY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Result of anti-CNPY3 (IP:15215-1-AP, 3ug; Detection:15215-1-AP 1:500) with mouse kidney tissue lysate 6000ug.
WB result of CNPY3 antibody (15215-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY3 transfected A549 cells.
Various lysates were subjected to SDS PAGE followed by western blot with 15215-1-AP (CNPY3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.