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OXA1L Monoclonal antibody
OXA1L Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
4A2D9
验证数据展示
经过测试的应用
| Positive WB detected in | T-47D cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, HepG2 cells, L02 cells, Jurkat cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
66128-1-Ig targets OXA1L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | OXA1L fusion protein Ag13819 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | oxidase (cytochrome c) assembly 1-like |
| Synonyms | Hsa, OXA1 like protein, OXA1Hs, OXA1L |
| Calculated Molecular Weight | 437 aa, 49 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC001669 |
| Gene Symbol | OXA1L |
| Gene ID (NCBI) | 5018 |
| RRID | AB_2881527 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15070 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OXA1L/Oxa1 is a member of the conserved Oxa1/YidC/Alb3 protein family and is required for cytochrome c oxidase assembly. The OXA1L protein was initially identified in yeast proteins involved in the biogenesis of the mitochondrial respiratory chain complexes. It locates in the inner membrane of mitochondria and may be involved in mediating the insertion of proteins from the matrix into the inner membrane.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OXA1L antibody 66128-1-Ig | Download protocol |
| IHC protocol for OXA1L antibody 66128-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter. | ||
