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CNTN2 Monoclonal antibody

CNTN2 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1D3G5

Cat no : 67089-1-Ig

Print datasheet

Synonyms

Axonal glycoprotein TAG 1, Axonin 1, AXT, CNTN2, Contactin 2, contactin 2 (axonal), DKFZp781D102, FLJ42746, TAG 1, TAG1, TAX, TAX 1, TAX1

验证数据展示

  • IHC staining of mouse brain using 67089-1-Ig

    IHC staining of mouse brain using 67089-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67089-1-Ig (CNTN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of pig brain using 67089-1-Ig

    WB analysis of pig brain using 67089-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67089-1-Ig (CNTN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 67089-1-Ig

    IF Staining of mouse brain using 67089-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CNTN2 antibody (67089-1-Ig, Clone: 1D3G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inpig brain tissue, pig cerebellum tissue, pig spinal cord tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67089-1-Ig targets CNTN2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human, pig, mouse
Immunogen CNTN2 fusion protein Ag28301 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name contactin 2 (axonal)
Synonyms Axonal glycoprotein TAG 1, Axonin 1, AXT, CNTN2, Contactin 2, contactin 2 (axonal), DKFZp781D102, FLJ42746, TAG 1, TAG1, TAX, TAX 1, TAX1
Calculated Molecular Weight 113 kDa
Observed Molecular Weight113-135 kDa
GenBank Accession NumberNM_005076
Gene Symbol CNTN2
Gene ID (NCBI) 6900
RRIDAB_2918481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02246
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CNTN2 antibody 67089-1-IgDownload protocol
IHC protocol for CNTN2 antibody 67089-1-IgDownload protocol
IF protocol for CNTN2 antibody 67089-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols