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SFPQ Monoclonal antibody
SFPQ Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
1G4A5
验证数据展示
经过测试的应用
| Positive WB detected in | U-251 cells, HSC-T6 cells, HeLa cells, Jurkat cells, PC-3 cells, HEK-293 cells, NIH/3T3 cells, A431 cells, LNCaP cells, K-562 cells |
| Positive IHC detected in | rat stomach tissue, human colon cancer tissue, human lung cancer tissue, human pancreas cancer tissue, mouse brain tissue, mouse stomach tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, MCF-7 cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy SFPQ Ready-To-Use IHC Kit. SFPQ primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
67129-1-Ig targets SFPQ in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | SFPQ fusion protein Ag7181 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated) |
| Synonyms | POMP100, POMP 100, Polypyrimidine tract-binding protein-associated-splicing factor, DNA-binding p52/p100 complex, 100 kDa subunit, 1G4A5 |
| Calculated Molecular Weight | 76 kDa |
| Observed Molecular Weight | 90-100 kDa |
| GenBank Accession Number | BC051192 |
| Gene Symbol | SFPQ |
| Gene ID (NCBI) | 6421 |
| RRID | AB_2882428 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P23246 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SFPQ, also named PSF, encodes a nuclear factor implicated in the splicing and regulation of gene expression. SFPQ probably forms a heteromer with NONO and participates in DNA pairing and DNA break repair program. Very recently SFPQ was identified as a downstream target of tau, complete nuclear depletion and cytoplasmic accumulation of SFPQ were shown in the neurons and astrocytes of brains with Alzheimer's disease (AD), more strikingly, reduced SFPQ levels may progress together with tau pathology, these observation strongly suggests the important role of SFPQ pathology in neurodegenerative diseases including AD. SFPQ encompasses 707 amino acids and has a molecular weight of 76 kDa, although it typically migrates on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel at an apparent molecular weight of ∼100 kDa. Proteolytic cleavage products of apparent molecular weights of 47 and 68 kDa, and an alternatively spliced form of 669 amino acids, have also been described in various cell types. (PMID: 25832716). Splicing Factor Proline and Glutamine rich (SFPQ) as the most significant intron-retaining transcript across diverse ALS-causing mutations (VCP, SOD1 and FUS). SFPQ protein binds extensively to its retained intron, which exhibits high cytoplasmic abundance in VCP mutation compared with controls. Crucially, the protein is less abundant in the nuclei of VCP mutation cultures and is ultimately lost from nuclei of MNs in mouse models (SOD1mu and VCP mutation transgenic mouse models) and human sporadic ALS post-mortem samples. In summary, our study implicates SFPQ IR and nuclear loss as general molecular hallmarks of familial and sporadic ALS.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SFPQ antibody 67129-1-Ig | Download protocol |
| IHC protocol for SFPQ antibody 67129-1-Ig | Download protocol |
| IF protocol for SFPQ antibody 67129-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Serum Splicing Factor Proline- and Glutamine-Rich Is a Diagnostic Marker for Non-Small-Cell Lung Cancer and Other Solid Cancers |
