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HPD Monoclonal antibody

HPD Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3C9G2

Cat no : 67552-1-Ig

Print datasheet

Synonyms

4 HPPD, 4HPPD, GLOD3, HPD, HPPDase, PPD

验证数据展示

  • WB analysis using 67552-1-Ig

    WB analysis using 67552-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67552-1-Ig (HPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67552-1-Ig

    IHC staining of human liver cancer using 67552-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67552-1-Ig (HPD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67552-1-Ig

    IHC staining of human liver cancer using 67552-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67552-1-Ig (HPD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, pig liver tissue, rat liver tissue, HepG2 cells, HEK-293 cells, HSC-T6 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67552-1-Ig targets HPD in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivityhuman, zebrafish
Immunogen HPD fusion protein Ag10928 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name 4-hydroxyphenylpyruvate dioxygenase
Synonyms 4 HPPD, 4HPPD, GLOD3, HPD, HPPDase, PPD
Calculated Molecular Weight 393 aa, 45 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC024287
Gene Symbol HPD
Gene ID (NCBI) 3242
RRIDAB_2882766
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP32754
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HPD antibody 67552-1-IgDownload protocol
IHC protocol for HPD antibody 67552-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,zebrafishWB

Pest Manag Sci

In vivo fluorescent screening for HPPD-targeted herbicide discovery

Authors - Yi-Xuan Fu
View Article
humanIHC

Life Sci

Homogentisic acid metabolism inhibits papillary thyroid carcinoma proliferation through ROS and p21-induced cell cycle arrest

Authors - Ruiwang Xie
View Article