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GMEB2 Monoclonal antibody

GMEB2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H10H8

Cat no : 68010-1-Ig

Print datasheet

Synonyms

DNA binding protein p79PIF, GMEB 2, GMEB2, KIAA1269, P79PIF, PIF p79, PIF79

验证数据展示

  • WB analysis using 68010-1-Ig

    WB analysis using 68010-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis using 68010-1-Ig

    WB analysis using 68010-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis using 68010-1-Ig

    WB analysis using 68010-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 68010-1-Ig

    WB analysis of MCF-7 using 68010-1-Ig

    WB result of GMEB2 antibody (68010-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GMEB2 transfected MCF-7 cells.

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, Jurkat cells, RAW 264.7 cells, HEK-293 cells, K-562 cells, PC-12 cells, HSC-T6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy GMEB2 Ready-To-Use IHC Kit. GMEB2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68010-1-Ig targets GMEB2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen GMEB2 fusion protein Ag31393 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glucocorticoid modulatory element binding protein 2
Synonyms DNA binding protein p79PIF, GMEB 2, GMEB2, KIAA1269, P79PIF, PIF p79, PIF79
Calculated Molecular Weight 530 aa, 56 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC036305
Gene Symbol GMEB2
Gene ID (NCBI) 26205
RRIDAB_2918757
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UKD1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GMEB-2 (glucocorticoid modulatory element-binding protein 2), also known as PIF79 (Parvovirus initiation factor p79) or P79PIF, is a DNA-binding protein that plays a crucial role in modulating transcription upon activation by steroid hormones. GMEB-2 is ubiquitously expressed with preferential expression in developmentally important tissues.

实验方案

Product Specific Protocols
WB protocol for GMEB2 antibody 68010-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols