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ATL3 Monoclonal antibody

ATL3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E7C4

Cat no : 68516-1-Ig

Print datasheet

Synonyms

EC:3.6.5.-, Atlastin-3, Atlastin 3, 2E7C4

验证数据展示

  • WB analysis using 68516-1-Ig

    WB analysis using 68516-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68516-1-Ig (ATL3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 68516-1-Ig

    WB analysis of Jurkat using 68516-1-Ig

    WB result of ATL3 antibody (68516-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATL3 transfected Jurkat cells.

经过测试的应用

Positive WB detected inPC-3 cells, Jurkat cells, HeLa cells, K-562 cells, rabbit testis tissue, rat testis tissue, mouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68516-1-Ig targets ATL3 in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat, rabbit
Immunogen ATL3 fusion protein Ag10248 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name atlastin GTPase 3
Synonyms EC:3.6.5.-, Atlastin-3, Atlastin 3, 2E7C4
Calculated Molecular Weight 541 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC077727
Gene Symbol ATL3
Gene ID (NCBI) 25923
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6DD88
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.

实验方案

Product Specific Protocols
WB protocol for ATL3 antibody 68516-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols