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CARHSP1 Recombinant antibody

CARHSP1 Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230489F3

Cat No : 83300-2-RR

Print datasheet

Synonyms

CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1

验证数据展示

  • IHC staining of mouse testis using 83300-2-RR

    IHC staining of mouse testis using 83300-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83300-2-RR (CARHSP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 83300-2-RR

    IF Staining of HepG2 using 83300-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CARHSP1 antibody (83300-2-RR, Clone: 230489F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HepG2 using 83300-2-RR

    FC experiment of HepG2 using 83300-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CARHSP1 Recombinant antibody (83300-2-RR, Clone:230489F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83300-2-RR is CARHSP1 Fusion Protein expressed in E. coli.

Tested Applications IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen CARHSP1 fusion protein Ag2265 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name calcium regulated heat stable protein 1, 24kDa
Synonyms CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1
Calculated Molecular Weight 147 aa, 16 kDa
GenBank Accession NumberBC003366
Gene Symbol CARHSP1
Gene ID (NCBI) 23589
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2V2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CARHSP1 antibody 83300-2-RRDownload protocol
IF protocol for CARHSP1 antibody 83300-2-RRDownload protocol
FC protocol for CARHSP1 antibody 83300-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols