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AGPAT2 Recombinant antibody, PBS Only (Capture)

AGPAT2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240153C2

Cat no : 83349-3-PBS

Print datasheet

Synonyms

240153C2, 1-AGPAT 2, 1-AGP acyltransferase 2, 1-acyl-sn-glycerol-3-phosphate acyltransferase beta, 1-acylglycerol-3-phosphate O-acyltransferase 2

验证数据展示

  • WB analysis using 83349-3-RR (same clone as 83349-3-PBS)

    WB analysis using 83349-3-RR (same clone as 83349-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83349-3-RR (AGPAT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83349-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83349-3-RR (same clone as 83349-3-PBS)

    IHC staining of human ovary cancer using 83349-3-RR (same clone as 83349-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83349-3-RR (AGPAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83349-3-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83349-3-RR (same clone as 83349-3-PBS)

    FC experiment of A549 using 83349-3-RR (same clone as 83349-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug AGPAT2 Recombinant antibody (83349-3-RR, Clone:240153C2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83349-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00360-2

    Cytometric bead array standard curve of MP00360-2

    Cytometric bead array standard curve of MP00360-2, AGPAT2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83349-3-PBS. Detection antibody: 83349-1-PBS. Standard: Ag34761. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83349-3-RR

    Affinity and Kinetic Characterization of 83349-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83349-3-RR against Human AGPAT2 were performed. The affinity constant is 2.72 nM.

产品信息

83349-3-PBS targets AGPAT2 as part of a matched antibody pair:

MP00360-2: 83349-3-PBS capture and 83349-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen AGPAT2 fusion protein Ag34761 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)
Synonyms 240153C2, 1-AGPAT 2, 1-AGP acyltransferase 2, 1-acyl-sn-glycerol-3-phosphate acyltransferase beta, 1-acylglycerol-3-phosphate O-acyltransferase 2
Calculated Molecular Weight 31 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC019292
Gene Symbol AGPAT2
Gene ID (NCBI) 10555
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15120
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

1-acyl-sn-glycerol-3-phosphate acyltransferase beta (AGPAT2) belongs to a family of enzymes catalyzing the sn-2 acylation of the glycerol-3-phosphate backbone. AGPAT2 is highly expressed in adipose tissues, liver, and skeletal muscle. AGPAT2 is the only AGPAT isoform whose loss-of-function mutations cause a severe form of human congenital generalized lipodystrophy (PMID: 34824276). Human and mouse AGPAT2 have a calculated molecular mass of 31 kDa, and an alternatively spliced form of AGPAT2 mRNA, encoding a protein of 246 rather than 278 amino acids, is also found in human (PMID: 19336658). Western detected AGPAT2 at an apparent molecular mass of 27 kDa.