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ALG9 Polyclonal antibody

ALG9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29218-1-AP

Print datasheet

Synonyms

EC:2.4.1.259, Dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase, Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase, DIBD1, Asparagine-linked glycosylation protein 9 homolog

验证数据展示

  • IHC staining of human stomach cancer using 29218-1-AP

    IHC staining of human stomach cancer using 29218-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29218-1-AP (ALG9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 29218-1-AP

    WB analysis using 29218-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29218-1-AP (ALG9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 29218-1-AP

    IHC staining of human stomach cancer using 29218-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29218-1-AP (ALG9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHL-60 cells, Jurkat cells, THP-1 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29218-1-AP targets ALG9 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen ALG9 fusion protein Ag30489 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name asparagine-linked glycosylation 9, alpha-1,2-mannosyltransferase homolog (S. cerevisiae)
Synonyms EC:2.4.1.259, Dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase, Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase, DIBD1, Asparagine-linked glycosylation protein 9 homolog
Calculated Molecular Weight 70 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC009255
Gene Symbol ALG9
Gene ID (NCBI) 79796
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6U8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALG9 encodes an endoplasmic reticulum enzyme that builds N-glycans, the third ER protein-encoding polycystic disease gene after GANAB and DNAJB11. Autosomal recessive loss of ALG9 results in a severe congenital disorder of glycosylation (CDG) with a multiorgan phenotype that includes kidney cysts (PMID: 31395617). Western blot analysis detected ALG9 at an apparent molecular mass of 70 kDa.

实验方案

Product Specific Protocols
WB protocol for ALG9 antibody 29218-1-APDownload protocol
IHC protocol for ALG9 antibody 29218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols