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C10orf28 Polyclonal antibody

C10orf28 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24967-1-AP

Print datasheet

Synonyms

Putative mitochondrial space protein 32.1, PSORT, Growth inhibition and differentiation-related protein 88, GIDRP88, GIDRP86

验证数据展示

  • WB analysis of A549 using 24967-1-AP

    WB analysis of A549 using 24967-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24967-1-AP (C10orf28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24967-1-AP

    IF Staining of HepG2 using 24967-1-AP

    Immunofluorescent analysis of HepG2 cells using 24967-1-AP (C10orf28 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human small intestine using 24967-1-AP

    IHC staining of human small intestine using 24967-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24967-1-AP (C10orf28 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inA549 cells, A431 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24967-1-AP targets C10orf28 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen C10orf28 fusion protein Ag21740 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 10 open reading frame 28
Synonyms Putative mitochondrial space protein 32.1, PSORT, Growth inhibition and differentiation-related protein 88, GIDRP88, GIDRP86
Calculated Molecular Weight 792 aa, 88 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC098359
Gene Symbol C10orf28
Gene ID (NCBI) 27291
RRIDAB_2879824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z5L2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C10orf28 antibody 24967-1-APDownload protocol
IHC protocol for C10orf28 antibody 24967-1-APDownload protocol
IF protocol for C10orf28 antibody 24967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols