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CARHSP1 Recombinant antibody

CARHSP1 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230489A2

Cat No : 83300-3-RR

Print datasheet

Synonyms

CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1

验证数据展示

  • WB analysis using 83300-3-RR

    WB analysis using 83300-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83300-3-RR (CARHSP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83300-3-RR

    FC experiment of HepG2 using 83300-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CARHSP1 Recombinant antibody (83300-3-RR, Clone:230489A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, HEK-293T cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83300-3-RR is CARHSP1 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen CARHSP1 fusion protein Ag2265 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name calcium regulated heat stable protein 1, 24kDa
Synonyms CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1
Calculated Molecular Weight 147 aa, 16 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC003366
Gene Symbol CARHSP1
Gene ID (NCBI) 23589
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y2V2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CARHSP1(calcium-regulated heat-stable protein 1, a 24 kDa protein also known as CRHSP-24) is a ubiquitously expressed phosphoprotein that is comprised of 147 amino acids with nearly 14% proline (PMID: 21990353,30257675). CARHSP1 was discovered as a biomarker for diabetic retinopathy and as a regulator of TNF-α mRNA stability in macrophages (PMID: 21078874). CARHSP1 may be involved in oxidative stress via traffic between stress granules and processing bodies (PMID: 21177848).

实验方案

Product Specific Protocols
WB protocol for CARHSP1 antibody 83300-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols