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DYRK4 Polyclonal antibody

DYRK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12890-2-AP

Print datasheet

Synonyms

DYRK4

验证数据展示

  • WB analysis of mouse lung using 12890-2-AP

    WB analysis of mouse lung using 12890-2-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 12890-2-AP

    IHC staining of human lymphoma using 12890-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 12890-2-AP (DYRK4 antibody) at dilution of 1:100 (under 10x lens).

经过测试的应用

Positive WB detected inmouse lung tissue, human brain tissue, Jurkat cells, mouse testis tissue, U-937 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12890-2-AP targets DYRK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DYRK4 fusion protein Ag3515 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4
Synonyms DYRK4
Calculated Molecular Weight 520 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC031244
Gene Symbol DYRK4
Gene ID (NCBI) 8798
RRIDAB_2094086
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR20
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dual-specificity tyrosine phosphorylation regulated kinase 4(DYRK4), belongs to a conserved family of serine/threonine protein kinases. DYRK4 is thought to function in the regulation of cell differentiation and proliferation, survival, and in development. DYRK4 has 5 isoforms with molecular weight of 59, 72 and 73 kDa.

实验方案

Product Specific Protocols
WB protocol for DYRK4 antibody 12890-2-APDownload protocol
IHC protocol for DYRK4 antibody 12890-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols