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ELP4 Polyclonal antibody

ELP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12746-1-AP

Print datasheet

Synonyms

C11orf19, Elongator complex protein 4, ELP4, hELP4, PAX6 neighbor gene protein, PAX6NEB, PAXNEB

验证数据展示

  • WB analysis of human cerebellum using 12746-1-AP

    WB analysis of human cerebellum using 12746-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 12746-1-AP (ELP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 12746-1-AP

    IHC staining of human endometrial cancer using 12746-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12746-1-AP (ELP4 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human endometrial cancer using 12746-1-AP

    IHC staining of human endometrial cancer using 12746-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12746-1-AP (ELP4 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman cerebellum tissue, human brain tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12746-1-AP targets ELP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ELP4 fusion protein Ag3707 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name elongation protein 4 homolog (S. cerevisiae)
Synonyms C11orf19, Elongator complex protein 4, ELP4, hELP4, PAX6 neighbor gene protein, PAX6NEB, PAXNEB
Calculated Molecular Weight 424 aa, 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC012514
Gene Symbol ELP4
Gene ID (NCBI) 26610
RRIDAB_2099745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EB1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ELP4 antibody 12746-1-APDownload protocol
IHC protocol for ELP4 antibody 12746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols