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FEZ2 Recombinant antibody

FEZ2 Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240248F5

Cat no : 83407-6-RR

Print datasheet

Synonyms

Zygin-2, Zygin II, Zygin 2, HUM3CL, Fasciculation and elongation protein zeta-2

验证数据展示

  • FC experiment of A431 using 83407-6-RR

    FC experiment of A431 using 83407-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Fez2 Recombinant Antibody (83407-6-RR, Clone:240248F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83407-6-RR

    Affinity and Kinetic Characterization of 83407-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83407-6-RR against Human FEZ2 were performed. The affinity constant is 6.64 nM.

经过测试的应用

Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83407-6-RR is FEZ2 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen FEZ2 fusion protein Ag15370 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fasciculation and elongation protein zeta 2 (zygin II)
Synonyms Zygin-2, Zygin II, Zygin 2, HUM3CL, Fasciculation and elongation protein zeta-2
Calculated Molecular Weight 353 aa, 40 kDa
GenBank Accession NumberBC110386
Gene Symbol FEZ2
Gene ID (NCBI) 9637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UHY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FEZ2 is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles.

实验方案

Product Specific Protocols
FC protocol for FEZ2 antibody 83407-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols