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KLHL13 Polyclonal antibody

KLHL13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15612-1-AP

Print datasheet

Synonyms

BKLHD2, kelch like 13 (Drosophila), Kelch like protein 13, KIAA1309, KLHL13

验证数据展示

  • WB analysis of Jurkat using 15612-1-AP

    WB analysis of Jurkat using 15612-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15612-1-AP (KLHL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 15612-1-AP

    IHC staining of human lung using 15612-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15612-1-AP (KLHL13 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 15612-1-AP

    IHC staining of mouse skeletal muscle using 15612-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15612-1-AP (KLHL13 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 15612-1-AP

    IHC staining of mouse skeletal muscle using 15612-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15612-1-AP (KLHL13 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells
Positive IHC detected inmouse skeletal muscle tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15612-1-AP targets KLHL13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KLHL13 fusion protein Ag8003 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch-like 13 (Drosophila)
Synonyms BKLHD2, kelch like 13 (Drosophila), Kelch like protein 13, KIAA1309, KLHL13
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC008729
Gene Symbol KLHL13
Gene ID (NCBI) 90293
RRIDAB_2130739
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2N7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL13 antibody 15612-1-APDownload protocol
IHC protocol for KLHL13 antibody 15612-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols