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MRPS6 Polyclonal antibody

MRPS6 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 16273-1-AP

Print datasheet

Synonyms

Small ribosomal subunit protein bS6m, S6mt, RPMS6, MRP-S6, MRP S6

验证数据展示

  • WB analysis using 16273-1-AP

    WB analysis using 16273-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16273-1-AP (MRPS6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16273-1-AP

    IF Staining of HepG2 using 16273-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPS6 antibody (16273-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16273-1-AP targets MRPS6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen MRPS6 fusion protein Ag9345 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S6
Synonyms Small ribosomal subunit protein bS6m, S6mt, RPMS6, MRP-S6, MRP S6
Calculated Molecular Weight 125 aa, 14 kDa
Observed Molecular Weight14 kDa
GenBank Accession NumberBC010076
Gene Symbol MRPS6
Gene ID (NCBI) 64968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP82932
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS6 antibody 16273-1-APDownload protocol
IF protocol for MRPS6 antibody 16273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols