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Recombinant Mouse IGFBP-3 protein (His Tag)

ED50

0.05-0.2 µg/mL

Species

Mouse

Purity

>95 %, SDS-PAGE

GeneID

16009

Accession

CAA57271.1

Cat No : Eg0513

Print datasheet

Synonyms

BP-53, IBP3, IGFBP3, IGFBP-3

验证数据展示

  • Purity of Recombinant Mouse IGFBP-3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse IGFBP-3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Measured by its ability to inhibit the biological activity of IGF-I or IGFII on MCF-7 human breast cancer cells. The ED50 for this effect is 0.05-0.2 µg/mL in the presence of 30 ng/mL Recombinant human IGF-I.

    Measured by its ability to inhibit the biological activity of IGF-I or IGFII on MCF-7 human breast cancer cells. The ED50 for this effect is 0.05-0.2 µg/mL in the presence of 30 ng/mL Recombinant human IGF-I.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Measured by its ability to inhibit the biological activity of IGF-I or IGFII on MCF-7 human breast cancer cells. The ED50 for this effect is 0.05-0.2 µg/mL in the presence of 30 ng/mL Recombinant human IGF-I.
Source HEK293-derived Mouse IGFBP-3 protein Pro22-Gln291 (Accession# CAA57271.1, Arg250Gln, Gln259Arg, Ser260Gly, Arg271Pro) with a His tag at the C-terminus.
Predicted Molecular Mass 30.4 kDa
SDS-PAGE 45-55 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein.

References:

1.Miljuš, G., Malenković, V., & Nedić, O. (2013).  Metallomics，5(3), 251–258.
2. Renes, J. S., van Doorn, J., & Hokken-Koelega, A. C. (2014). J Clin Endocrinol Metab, 99(10):E1988-E1996. 
3.Jia Y, Lee KW, Swerdloff R, et al. (2010). J Biol Chem, 285(3), 1726–1732.