• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RHOA Polyclonal antibody, PBS Only

RHOA Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27213-1-PBS

Print datasheet

Synonyms

ARHA, ARH12, RHO12, Rho cDNA clone 12, h12

验证数据展示

  • WB analysis of mouse kidney using 27213-1-AP (same clone as 27213-1-PBS)

    WB analysis of mouse kidney using 27213-1-AP (same clone as 27213-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27213-1-AP (RHOA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27213-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 27213-1-AP (same clone as 27213-1-PBS)

    IHC staining of human stomach using 27213-1-AP (same clone as 27213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27213-1-AP (RHOA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27213-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RHOA antibody included
Planning an IHC experiment? We recommend our IHCeasy RHOA Ready-To-Use IHC Kit. RHOA primary antibody included.

产品信息

27213-1-PBS targets RHOA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen RHOA fusion protein Ag25873 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ras homolog gene family, member A
Synonyms ARHA, ARH12, RHO12, Rho cDNA clone 12, h12
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC005976
Gene Symbol RHOA
Gene ID (NCBI) 387
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61586
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.