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CD138/Syndecan-1 Polyclonal antibody

CD138/Syndecan-1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 10593-1-AP

Print datasheet

Synonyms

CD138, CD138/Syndecan-1, SDC, SDC1, SYND1, syndecan, syndecan 1



经过测试的应用

Positive WB detected inA549 cells, mouse spleen tissue, HeLa cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD138/SDC1 Ready-To-Use IHC Kit. CD138/SDC1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10593-1-AP targets CD138/Syndecan-1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Immunogen CD138/Syndecan-1 fusion protein Ag0823 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name syndecan 1
Synonyms CD138, CD138/Syndecan-1, SDC, SDC1, SYND1, syndecan, syndecan 1
Calculated Molecular Weight 32.5 kDa
Observed Molecular Weight60-100 kDa, 130 kDa
GenBank Accession NumberBC008765
Gene Symbol SDC1
Gene ID (NCBI) 6382
RRIDAB_2182736
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18827
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas. CD138 has a calculated molecular weight of 32.5 kDa, the apparent molecular weight is higher due to extensive glycosylation.

实验方案

Product Specific Protocols
WB protocol for CD138/Syndecan-1 antibody 10593-1-APDownload protocol
IHC protocol for CD138/Syndecan-1 antibody 10593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell

Tumor-reactive antibodies evolve from non-binding and autoreactive precursors.

Authors - Roei D Mazor
humanWB,IP

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
mouseIHC

Leukemia

miR-21 antagonism abrogates Th17 tumor promoting functions in multiple myeloma.

Authors - Marco Rossi
mouseIF

Theranostics

Aurka loss in CD19+ B cells promotes megakaryocytopoiesis via IL-6/STAT3 signaling-mediated thrombopoietin production.

Authors - Xin Chen

Mol Neurodegener

Amelioration of amyloid-β-induced deficits by DcR3 in an Alzheimer's disease model.

Authors - Yi-Ling Liu
humanWB

Cell Mol Life Sci

The association of telomere maintenance and TERT expression with susceptibility to human papillomavirus infection in cervical epithelium.

Authors - Li-Na Wang