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SLC26A3 Polyclonal antibody

SLC26A3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 13165-1-AP

Print datasheet

Synonyms

DRA, Down-regulated in adenoma, CLD, Chloride anion exchanger

验证数据展示

  • IHC staining of human colon cancer using 13165-1-AP

    IHC staining of human colon cancer using 13165-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 13165-1-AP (SLC26A3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 13165-1-AP

    IHC staining of human colon using 13165-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13165-1-AP (SLC26A3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 13165-1-AP

    IHC staining of human colon using 13165-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13165-1-AP (SLC26A3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 13165-1-AP

    IHC staining of mouse testis using 13165-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13165-1-AP (SLC26A3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 13165-1-AP

    IHC staining of mouse testis using 13165-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13165-1-AP (SLC26A3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse testis tissue, human colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13165-1-AP targets SLC26A3 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SLC26A3 fusion protein Ag3816 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 26, member 3
Synonyms DRA, Down-regulated in adenoma, CLD, Chloride anion exchanger
Calculated Molecular Weight 764 aa, 85 kDa
Observed Molecular Weight84 kDa
GenBank Accession NumberBC025671
Gene Symbol SLC26A3
Gene ID (NCBI) 1811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP40879
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC26A3 (Chloride anion exchanger), also known as DRA. It is expected to be located in the cytoplasm and cell membranes. It is a transmembrane glycoprotein, which is mainly located in the lower digestive tract mucosa, especially in the apical membrane of columnar epithelium and some goblet cells. The expression level of SLC26A3 in intestine is different along different parts of intestine, and there are also differences on crypt-villus axis. It is mainly expressed in the apical membrane of colon epithelial cells, less in jejunum and ileum, but not in esophagus. It participates in the absorption of electrically neutral NaCl in intestine and works with Na+/H+ exchanger. Cl-/HCO3- exchange mediated by SLC26A3 is very important for maintaining intestinal acid-base balance. In duodenum, the physiological function of SLC26A3 is related to HCO3- secretion, which plays a central role in neutralizing gastric acid (PMID: 32989468). The molecular weight of SLC26A3 is 84 kDa.

实验方案

Product Specific Protocols
IHC protocol for SLC26A3 antibody 13165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols