CoraLite®594-conjugated SQLE Monoclonal antibody
SQLE Monoclonal Antibody for
Host / Isotype
Mouse / IgG2a
Reactivity
human, rat, pig
Applications
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1C9A2
验证数据展示
For other applications, we recommend the unconjugated version of this antibody, 67206-1-Ig
产品信息
CL594-67206 targets SQLE in applications and shows reactivity with human, rat, pig samples.
Tested Applications | Application Description |
Tested Reactivity | human, rat, pig |
Immunogen | SQLE fusion protein Ag3266 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | squalene epoxidase |
Synonyms | Squalene monooxygenase, squalene epoxidase, ERG1, EC:1.14.14.17, 1C9A2 |
Calculated Molecular Weight | 574 aa, 64 kDa |
Observed Molecular Weight | 50-64 kDa |
GenBank Accession Number | BC017033 |
Gene Symbol | SQLE |
Gene ID (NCBI) | 6713 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q14534 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation. SQLE is subject to feedback regulation via cholesterol-induced degradation, which depends on its lipid-sensing N terminal regulatory domain. Truncation of SQLE occurs during its endoplasmic reticulum-associated degradation and requires the proteasome, which partially degrades the SQLE N-terminus and eliminates cholesterol-sensing elements within this region. The MW of SQLE is about 50-64 kDa. (PMID:21356516, PMID: 28972164)