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TAP1 Polyclonal antibody
TAP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
IF, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HCT 116 cells, HepG2 cells, mouse skeletal muscle tissue, HeLa cells, mouse spleen tissue, SW480 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11114-1-AP targets TAP1 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF, IHC, IP, WB, ELISA |
Cited Applications | IF, IHC, IP, RIP, WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | TAP1 fusion protein Ag1619 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) |
Synonyms | ABCB2, Antigen peptide transporter 1, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter TAP1, PSF 1, PSF1, RING 4, RING4, TAP1, transporter associated with antigen 1 processing 1 |
Calculated Molecular Weight | 81 kDa |
Observed Molecular Weight | 70-81 kDa |
GenBank Accession Number | BC014081 |
Gene Symbol | TAP1 |
Gene ID (NCBI) | 6890 |
RRID | AB_2200201 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q03518 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TAP1 antibody 11114-1-AP | Download protocol |
IHC protocol for TAP1 antibody 11114-1-AP | Download protocol |
IF protocol for TAP1 antibody 11114-1-AP | Download protocol |
IP protocol for TAP1 antibody 11114-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Gastroenterology microRNA 125a Regulates MHC-I Expression on Esophageal Adenocarcinoma Cells, Associated With Suppression of Antitumor Immune Response and Poor Outcomes of Patients. | ||
Nat Commun Proteomic analysis of archival breast cancer clinical specimens identifies biological subtypes with distinct survival outcomes. | ||
Theranostics Nintedanib enhances the efficacy of PD-L1 blockade by upregulating MHC-I and PD-L1 expression in tumor cells. | ||
Int J Mol Sci Antigen Peptide Transporter 1 (TAP1) Promotes Resistance to MEK Inhibitors in Pancreatic Cancers. | ||
J Immunol Inducible TAP1 Negatively Regulates the Antiviral Innate Immune Response by Targeting the TAK1 Complex.
| ||
J Biol Chem The unfolded protein response (UPR)-activated transcription factor X-box-binding protein 1 (XBP1) induces microRNA-346 expression that targets the human antigen peptide transporter 1 (TAP1) mRNA and governs immune regulatory genes. |