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  • Featured Product
  • KD/KO Validated

TRAF4 Monoclonal antibody, PBS Only

TRAF4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C9A11

Cat no : 66755-1-PBS

Print datasheet

Synonyms

CART1, MLN 62, MLN62, RING finger protein 83, RNF83, TRAF4

验证数据展示

  • WB analysis using 66755-1-Ig (same clone as 66755-1-PBS)

    WB analysis using 66755-1-Ig (same clone as 66755-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 66755-1-Ig (same clone as 66755-1-PBS)

    IHC staining of human urothelial carcinoma using 66755-1-Ig (same clone as 66755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 66755-1-Ig (same clone as 66755-1-PBS)

    IHC staining of human urothelial carcinoma using 66755-1-Ig (same clone as 66755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66755-1-Ig (same clone as 66755-1-PBS)

    IF Staining of HeLa using 66755-1-Ig (same clone as 66755-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66755-1-Ig (TRAF4 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • WB analysis using 66755-1-Ig (same clone as 66755-1-PBS)

    WB analysis using 66755-1-Ig (same clone as 66755-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

产品信息

66755-1-PBS targets TRAF4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, pig
Immunogen TRAF4 fusion protein Ag27221 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TNF receptor-associated factor 4
Synonyms CART1, MLN 62, MLN62, RING finger protein 83, RNF83, TRAF4
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 50-54 kDa
GenBank Accession NumberBC001769
Gene Symbol TRAF4
Gene ID (NCBI) 9618
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BUZ4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The tumor necrosis factor receptor-associated factor (TRAF) family, consisting of seven members (TRAF1-7), is a group of signaling adaptors which participate in various signaling pathways through binding of the tumor necrosis factor receptor superfamily. As one of the important member of TRAF family proteins, TRAF4 was initially isolated from breast carcinomas and identified as the first member of the TRAF family to be up-regulated in human carcinomas. TRAF4 is required during embryogenesis in key biological processes including the formation of the trachea, the development of the axial skeleton, and the closure of the neural tube. TRAF4 deficiency leads to severe developmental alterations.