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PDI Monoclonal antibody, PBS Only (Capture)

PDI Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E6A11

Cat no : 66422-1-PBS

Print datasheet

Synonyms

P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1

验证数据展示

  • IF Staining of HepG2 using 66422-1-Ig (same clone as 66422-1-PBS)

    IF Staining of HepG2 using 66422-1-Ig (same clone as 66422-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDI antibody (66422-1-Ig, Clone: 2E6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • WB analysis using 66422-1-Ig (same clone as 66422-1-PBS)

    WB analysis using 66422-1-Ig (same clone as 66422-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66422-1-Ig (PDI Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

    IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDI/P4HB antibody included
  • IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

    IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDI/P4HB antibody included
  • Cytometric bead array standard curve of MP50798-2

    Cytometric bead array standard curve of MP50798-2

    Cytometric bead array standard curve of MP50798-2, PDI Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66422-1-PBS. Detection antibody: 66422-3-PBS. Standard:Ag1747. Range: 0.098-1.563 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy PDI/P4HB Ready-To-Use IHC Kit. PDI/P4HB primary antibody included.

产品信息

66422-1-PBS targets PDI as part of a matched antibody pair:

MP50798-2: 66422-1-PBS capture and 66422-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen PDI fusion protein Ag1747 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name prolyl 4-hydroxylase, beta polypeptide
Synonyms P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC014504
Gene Symbol PDI
Gene ID (NCBI) 5034
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07237
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).