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ACOX1 Recombinant antibody, PBS Only (Detector)

ACOX1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240754E3

Cat no : 83731-2-PBS

Print datasheet

Synonyms

Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX

验证数据展示

  • WB analysis using 83731-2-RR (same clone as 83731-2-PBS)

    WB analysis using 83731-2-RR (same clone as 83731-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83731-2-RR (ACOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83731-2-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83731-2-RR (same clone as 83731-2-PBS)

    FC experiment of A431 using 83731-2-RR (same clone as 83731-2-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ACOX1 Recombinant antibody (83731-2-RR, Clone:240754E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83731-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00709-3

    Sandwich ELISA standard curve of MP00709-3

    Sandwich ELISA standard curve of MP00709-3, Human AOX Recombinant Matched Antibody Pair - PBS only. 83731-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1405. 83731-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Sample test of MP00709-3

    Sample test of MP00709-3

    K-562 and U-251 cell lysates were measured. The human AOX concentration of detected samples was determined to be 158.79 ng/mL (based on a 2.1 mg/mL extract load) in K-562 cell lyaste and 345.46 ng/mL (based on a 1.6 mg/mL extract load) in U-251 cell lysate.

  • Cytometric bead array standard curve of MP00709-2

    Cytometric bead array standard curve of MP00709-2

    Cytometric bead array standard curve of MP00709-2, AOX Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83731-3-PBS. Detection antibody: 83731-2-PBS. Standard: Ag1405. Range: 1.56-100 ng/mL.

  • Cytometric bead array standard curve of MP00709-3

    Cytometric bead array standard curve of MP00709-3

    Cytometric bead array standard curve of MP00709-3, AOX Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83731-1-PBS. Detection antibody: 83731-2-PBS. Standard: Ag1405. Range: 0.78-50 ng/mL.

  • Affinity and Kinetic Characterization of 83731-2-RR

    Affinity and Kinetic Characterization of 83731-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83731-2-RR against Human ACOX1 were performed. The affinity constant is below 1 pM.

产品信息

83731-2-PBS targets ACOX1 as part of a matched antibody pair:

MP00709-2: 83731-3-PBS capture and 83731-2-PBS detection (validated in Cytometric bead array)

MP00709-3: 83731-1-PBS capture and 83731-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse, rat
Immunogen ACOX1 fusion protein Ag1405 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name acyl-Coenzyme A oxidase 1, palmitoyl
Synonyms Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX
Calculated Molecular Weight 74 kDa
Observed Molecular Weight70-74 kDa, 22 kDa
GenBank Accession NumberBC010425
Gene Symbol ACOX1
Gene ID (NCBI) 51
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15067
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).