mouse testis tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IF result (localization to the spindle poles) of anti-IFT81 (11744-1-AP, 1:50) with metaphase hTERT-RPE1 cells (MeOH fixed) byDr. Moshe Kim.
Immunofluorescent images of MDCK cells stained with IFT81 rabbit pAb (11744-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for IFT81, and Rhodamine-Goat anti-rabbit IgG for 66200-1-Ig.
IF result (the base and tip of cilia) of anti-IFT81 (11744-1-AP, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim.
IP Result of anti-IFT81 (IP:11744-1-AP, 3ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 7500ug.
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11744-1-AP (IFT81 antibody) at dilution of 1:50 (under 10x lens).
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).
Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT81 antibody (11744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).