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  • KD/KO Validated

14-3-3 Polyclonal antibody

14-3-3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 14503-1-AP

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Synonyms

14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 1C5, HS1, Protein HS1, YWHAQ



经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue, mouse brain tissue, Jurkat cells, rat liver tissue, mouse heart tissue, NIH/3T3 cells, C6 cells
Positive IP detected inmouse lung tissue
Positive IHC detected inmouse brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

产品信息

14503-1-AP targets 14-3-3 in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications CoIP, IHC, IP, RIP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen 14-3-3 fusion protein Ag5959 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Synonyms 14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 1C5, HS1, Protein HS1, YWHAQ
Calculated molecular weight 28 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC056867
Gene symbol YWHAQ
UniprotP27348
Gene ID (NCBI) 10971
RRIDAB_2218096
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

14-3-3 proteins are the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

实验方案

Product Specific Protocols
WB protocol for 14-3-3 antibody 14503-1-APDownload protocol
IHC protocol for 14-3-3 antibody 14503-1-APDownload protocol
IF protocol for 14-3-3 antibody 14503-1-APDownload protocol
IP protocol for 14-3-3 antibody 14503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Int J Biol Macromol

Biofilm formation initiating rotifer-specific biopolymer and its predicted components

Authors - Zsolt Datki
humanWB

Elife

Ser/Thr kinase Trc controls neurite outgrowth in Drosophila by modulating microtubule-microtubule sliding.

Authors - Rosalind Norkett
  • KD Validated
humanCoIP

Int J Mol Sci

APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.

Authors - Marianna Naki
human,mouseWB,IP

Aging (Albany NY)

The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.

Authors - Chunyan Li
humanWB

J Inflamm Res

PADI6 Regulates Trophoblast Cell Migration-Invasion Through the Hippo/YAP1 Pathway in Hydatidiform Moles.

Authors - Bo Huang
ratIP

Biochim Biophys Acta Mol Basis Dis

Nedd4-2 haploinsufficiency in mice causes increased seizure susceptibility and impaired Kir4.1 ubiquitination.

Authors - Xiaoliang Liu