免疫印迹
Proteintech和ChromeTek为您提供以快速和可靠的方式获得值得发表结果的解决方案。
一抗
多克隆抗体
识别感兴趣蛋白质的多个表位。具有非常高的亲和力。适合于低丰度的靶点，罕见物种和变异的蛋白质。 Proteintech的多克隆抗体来源于兔。
Top Cited Antibodies
GAPDH Monoclonal Antibody
| Reactivity: | Human, Mouse, Rat, Yeast, Plant, Zebrafish, Bovine and more (9) |
| Applications: | WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA |
TDP-43 Polyclonal Antibody
| Reactivity: | Human, Mouse, Rat, Zebrafish, Hamster, Pig and more (6) |
| Applications: | WB, RIP, IP, IHC, IF, IEM, FC, CoIP, chIP, ELISA |
GAPDH Polyclonal antibody
| Reactivity: | Human, Mouse, Rat, Pig, Arabidopsis, Corn, Cabbage, Rice and more (9) |
| Applications: | WB, RIP, IP, IHC, IF, FC, CoIP, ELISA |
Beta Actin Monoclonal antibody
| Reactivity: | Human, Mouse, Rat, Pig, Plant, Zebrafish And More (10) |
| Applications: | WB, IP, IHC, IF, FC, CoIP, ELISA |
BAX Polyclonal antibody
| Reactivity: | Human, Mouse, Rat, Chicken, Goat, Hamster, Pig, Rabbit and more (3) |
| Applications: | WB, IP, IHC, FC, CoIP, chIP, ELISA |
Caspase 3/p17/p19 Polyclonal antibody
| Reactivity: | Human, Mouse, Rat, Bovine, Chicken, Duck, Goat, Hamster and more (3) |
| Applications: | WB, RIP, IP, IHC, IF, FC, ELISA |
E-cadherin Polyclonal antibody
| Reactivity: | Human, Mouse, Rat, Bovine, Pig, Zebrafish |
| Applications: | WB. IF, WB, IP, IHC, IF, FC, CoIP, ELISA |
NF-κB p65 Polyclonal antibody
| Reactivity: | Human, Mouse, Rat, Bovine, Pig, Canine, Fish and more (4) |
| Applications: | WB, IP, IHC, IF, FC, chIP, ELISA |
Vimentin Polyclonal antibody
| Reactivity: | Human, Mouse, Rat, Bovine, Pig, Deer |
| Applications: | WB, IP, IHC, IF, FC, CoIP, ELISA |
P62,SQSTM1 Polyclonal antibody
| Reactivity: | Human, Bovine, Chicken, Goat, Hamster, Pig, Rabbit and more (3) |
| Applications: | WB, IP, IHC, IF, FC, CoIP, ELISA |
MMP9 (N-terminal) Polyclonal antibody
| Reactivity: | Human, Mouse, Rat, Pig, Rabbit, Hamster, Astragalus Membranaceus |
| Applications: | WB, IP, IHC, IF, FC, CoIP, ELISA |
Alpha Tubulin Polyclonal antibody
| Reactivity: | Human, Mouse, Rat, Bovine, Chicken, Goat, Hamster, Monkey, P. Lividus, Pig |
| Applications: | WB, IP, IHC, IF, FC, CoIP, ELISA |
ARL13B Polyclonal antibody
| Reactivity: | Human, Mouse, Rat, Canine, Chicken, Pig, Sheep, Xenopus, Zebrafish |
| Applications: | WB, IP, IHC, IF, ELISA |
PARK2/Parkin Polyclonal antibody
| Reactivity: | Human, Mouse, Rat, Pig, Canine, Chicken, Hamster |
| Applications: | WB, IP, IHC, IF, FC, ChIP, ELISA |
P53 Polyclonal antibody
| Reactivity: | Human, Rat, Mouse, Goat, Pig and more (3) |
| Applications: | WB, IP, IF, CoIP, chIP, ELISA |
二抗
选择WB二抗时，请注意：
- 一抗的宿主
- 您的信号检测系统
Popular and highly cited secondary antibodies for WB
Affinipure Goat Anti-Rabbit IgG (H+L)
| Conjugate: | HRP |
| Applications: | ELISA, WB |
Affinipure Goat Anti-Mouse IgG (H+L)
| Conjugate: | HRP |
| Applications: | ELISA, WB |
Affinipure Rabbit Anti-Goat IgG (H+L)
| Conjugate: | HRP |
| Applications: | ELISA, WB |
纳米二抗
与传统二抗相比，纳米二抗更具有优势。
- 纳米二抗与一抗的同时孵育，即一步孵育，减少了孵育时间，提高了多重使用的能力。
- 开发纳米二抗的重组过程确保了批次间的高度一致性。
- 更小尺寸的纳米二抗已被证明可以提高超分辨率显微技术中的图像分辨率。
Multiplexed immunostaining of HeLa cells with two alpaca anti-mouse Nano-Secondaries and one anti-rabbit Nano-Secondary. Green: mouse IgG1 anti-COX4 + alpaca anti-mouse IgG1 VHH Alexa Fluor 488. Magenta: mouse IgG2b anti-Tubulin + alpaca anti-mouse IgG2b VHH Alexa Fluor 647. Yellow: rabbit anti-Lamin + alpaca anti-rabbit IgG VHH Alexa Fluor 568. Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
化学发光底物
SignalBright®增强型化学发光底物 - 基于鲁米诺的化学发光底物，用于HRP（辣根过氧化物酶），比传统的ECL试剂提供更高的灵敏度。Proteintech有三种试剂可供选择，可以检测低至高飞克水平级的蛋白质。
Proteintech的SignalBright系列具有竞争产品无法比拟的性能，其检测灵敏度高，所需优化最少，以及长而稳定的信号持续时间。
Comparison of Proteintech’s SignalBright Pro (PK10011), SignalBright Plus (PK10012), and SignalBright Max (PK10013) Enhanced Chemiluminescent Substrate. Serial dilutions of HeLa cell lysate. Primary antibody: Proteintech Beta Catenin 51067-2-AP, 1:20,000. Secondary: Quanta BioDesign HRP-Goat anti-Rabbit IgG (H&L) (11-0201-0503) at a 1:100,000 dilution. Exposure Time: 30 seconds.
蛋白Marker
三种颜色的即用蛋白质分子量标准，是免疫印迹实验的完美伴侣。
Prestained protein marker
| Molecular Weight: | 10-180 kDa |
| Number of markers: | 10 |
Broad range prestained protein marker
| Molecular Weight: | 3-245 kDa |
| Number of markers: | 13 |
Extra Range Prestained Protein Marker
| Molecular Weight: | 10-310 kDa |
| Number of markers: | 13 |
免疫印迹的技术支持
免疫印迹是一种非常常见的应用。然而，由于免疫印迹流程的许多方面可能会干扰实验结果，因此得到好的免疫印迹数据可能并不容易。
我们的技术团队已经编写了一份出色的免疫印迹实验指南，详细描述了完整的实验方案和一个全面的问题排除部分。请点击这里下载。
