Lamin B1 Antibody 96 Publications

Rabbit Polyclonal| Catalog number: 12987-1-AP

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规格

单价

100 μl

¥980

500 μl

¥4000

1000 μl

¥6000


Species specificity:
human, mouse, rat, hamster, zebrafish

Positive WB detected in:
HeLa cells, NIH/3T3 cells, Y79 cells, K-562 cells, SH-SY5Y cells, mouse eye tissue, mouse kidney tissue, mouse lung tissue, mouse brain tissue, U-937 cells, rat brain tissue, rat heart tissue, Jurkat cells, mouse spleen tissue, HepG2 cells, human kidney tissue, mouse pancreas tissue, human brain tissue

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human breast cancer tissue, human kidney tissue

Positive IF detected in:
HepG2 cells, human skin cancer tissue, NIH/3T3 cells

Positive FC detected in:
HEK-293 cells

Recommended dilution:
WB : 1:1000-1:6000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
lamin B1

Calculated molecular weight:
66 kDa

Observed molecular weight:
66-70 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
LMNB1

Synonyms
ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1
Background

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis(PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.


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