Our antibodies are under guarantee for 1 year after purchasing. However, internal studies show that the antibody will remain functional for a minimum of five years when properly stored.
Please store the antibody in the original tube at -20°C and do not aliquot. Our antibodies are stored in 50% glycerol which prevents them from freezing.
You can find the detailed information for each antibody on their product datasheet. Please search and click the antibody of your interest to be directed to the datasheet for your antibody which includes validated applications, images, etc. If you would like to print the datasheet, click the print icon below the Catalog Number.
The concentration of a given antibody is found on the individual datasheet.
On the product page of your antibody, please click the link beside ‘Immunogen’ to view a description of the immunogen. Should you require more detailed information, please speak to a member of staff.
We use full-length recombinant proteins as immunogens to generate our antibodies that can be used as blocking reagents. However, we do not test the neutralizing effect of the antigen (the recombinant protein) and do not have any other blocking peptides against our antibodies.
We test our antibodies on human and mouse cells or tissues. To find information on other species, please contact us.
This depends on the extent of protein sequence similarity between the immunogen and the potential cross-reactive protein sequence. A pair-wise sequence alignment can be performed online through NCBI-BLAST website (Link to http://blast.ncbi.nlm.nih.gov/Blast.cgi).
Each product pages shows the number of publications and has links to the publication, if the antibody has already been cited.
Several reasons can cause a difference in band size, such as multimers, gel migration, relative charges, splice variants, post-translational modification or cleavage. For more information, please contact our tech support.
Each monoclonal antibody has a clone number that is specific for its single clone of hybridoma cells. The clone number can be found on the product page next to the catalog number.
We usually add 6x His tags or GST tags to the N-terminus of proteins and these proteins are then purified by Ni2+ and glutathione affinity chromatography respectively.
The His-tagged recombinant protein is usually stored in 1x PBS buffer (58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH 7.4) containing 300mM imidazole.
The GST-tagged recombinant protein is usually stored in 1x PBST buffer (58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, 1% Triton-X100, pH 7.4) containing 100mM glutathione (GSH).
Antibody-coated 96-well microplate, assay standard, detection antibody, HRP-conjugated antibody, sample diluent, detection diluent, wash buffer, tetramethylbenzidine (TMB) substrate, stop solution, and plate cover seals.
All Proteintech ELISA kits are sandwich ELISAs.
Yes, all plates are pre-coated with an antibody that is specific for the target.
It is recommended to run the experiment in duplicate at a minimum.
No, different lot numbers cannot be mixed.
Incubation times should be followed exactly as stated in the manual.
Please follow the instructions in the manual.
Most of our ELISA kits are validated for cell culture supernatant, plasma, or serum.
The ELISA sensitivity is highlighted in the data sheet for each kit.
There are multiple explanations: incorrect storage, incorrect incubation time, improper antibody, insufficient washing, etc. For more information, please contact technical support.
Improper washing, contaminated reagents, samples, or wells. For more information, please contact technical support.