6*His, His-tag Polyclonal antibody
6*His, His-tag Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein and More (5)
Applications
WB, ELISA and More (4)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | recombinant protein |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10001-0-AP targets 6*His, His-tag in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, ChIP |
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, rat, pig, monkey |
| Immunogen | Recombinant protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | 6*His, His-tag |
| Synonyms | 6*his,His tag, 6*His, 6 his, his tag, his |
| Calculated Molecular Weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_11232228 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for 6*His, His-tag antibody 10001-0-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
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